GRP94 Monoklonaler Antikörper

GRP94 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

4G7C7

Rezensionen (1)

Kat-Nr. : 60012-2-Ig

Synonyme

HSP90B1, 4G7C7, 94 kDa glucose-regulated protein, ECGP, Endoplasmin

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  WB analysis using 60012-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60012-2-Ig (GRP94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 60012-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60012-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60012-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60012-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60012-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP94 antibody (60012-2-Ig, Clone: 4G7C7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of A431 using 60012-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 60012-2-Ig (GRP94 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 60012-2-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (60012-2-Ig, Clone:4G7C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 60012-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 60012-2-Ig against Human GRP94 were performed. The affinity constant is 90.3 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

60012-2-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA GRP94 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag1439
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC009195
• Gene symbol: GRP94
• Gene ID (NCBI): 7184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Publikationen

Species	Application	Title
mouse	WB	J Control Release; Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility; Authors - Yabing Chen
human	WB	Anticancer Drugs; Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78.; Authors - Chenxue Gong
mouse	WB	Biomol Biomed; Exogenous spermidine alleviates diabetic cardiomyopathy via suppressing reactive oxygen species, endoplasmic reticulum stress, and Pannexin-1-mediated ferroptosis; Authors - Jian Sun
Human		Int J Mol Sci; Size-Exclusion Chromatography: A Path to Higher Yield and Reproducibility Compared to Sucrose Cushion Ultracentrifugation for Extracellular Vesicle Isolation in Multiple Myeloma; Authors - Madalena Grenhas
		Haematologica; DNAJC10 maintains survival and self-renewal of leukemia stem cells through PERK branch of the unfolded protein response; Authors - Minjing Li
human	WB	J Transl Med; Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration; Authors - Lingzi Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MSCs

GRP94 Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:60012-2-Ig)