GRP94 Monoklonaler Antikörper

GRP94 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G7C7

Kat-Nr. : 60012-2-PBS

Synonyme

HSP90B1, 4G7C7, 94 kDa glucose-regulated protein, ECGP, Endoplasmin

---

• 

  WB analysis using 60012-2-Ig (same clone as 60012-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60012-2-Ig (GRP94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50013-1

  Sandwich ELISA standard curve of MP50013-1, GRP94 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60012-2-PBS. Detection antibody: HRP-conjugated 60012-1-PBS. Standard: Ag1439. Range: 0.156-20 ng/mL..

• 

  IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP94 antibody (60012-2-Ig, Clone: 4G7C7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 60012-2-Ig (GRP94 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 60012-2-Ig (same clone as 60012-2-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (60012-2-Ig, Clone:4G7C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 60012-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 60012-2-Ig against Human GRP94 were performed. The affinity constant is 90.3 pM.

---

Geprüfte Anwendungen

Produktinformation

60012-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA GRP94 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag1439
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC009195
• Gene symbol: GRP94
• Gene ID (NCBI): 7184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).