GRP94 Rekombinanter Antikörper

GRP94 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6G15

Kat-Nr. : 82805-2-PBS

Synonyme

HSP90B1, 6G15, 94 kDa glucose-regulated protein, ECGP, Endoplasmin

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  WB analysis using 82805-2-RR (same clone as 82805-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82805-2-RR (GRP94 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 82805-2-RR (same clone as 82805-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82805-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82805-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82805-2-RR against Human GRP94 were performed. The affinity constant is 101 nM.

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Geprüfte Anwendungen

Produktinformation

82805-2-PBS bindet in WB, IHC, Indirect ELISA GRP94 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag6344
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC066656
• Gene symbol: GRP94
• Gene ID (NCBI): 7184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.