GRP94 Rekombinanter Antikörper

GRP94 Rekombinant Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

6G15

Kat-Nr. : CL750-82805-2

Synonyme

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

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  WB analysis using CL750-82805-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82805-2 (GRP94 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-82805-2 bindet in WB GRP94 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag6344
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC066656
• Gene symbol: GRP94
• Gene ID (NCBI): 7184
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.