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GRWD1 Rekombinanter Antikörper

GRWD1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251273F6

Kat-Nr. : 86451-3-PBS

Synonyme

Glutamate-rich WD repeat-containing protein 1, GRWD, KIAA1942, RRB1, WDR28

Filter:
  • Western Blot (WB) analysis of various lysates using GRWD1 Recombinant antibody (86451-3-RR)

    WB analysis using 86451-3-RR (same clone as 86451-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86451-3-RR (GRWD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86451-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GRWD1 Recombinant antibody (86451-3-RR)

    IF Staining of HeLa using 86451-3-RR (same clone as 86451-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86451-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRWD1 Recombinant antibody (86451-3-RR)

    IF Staining of HepG2 using 86451-3-RR (same clone as 86451-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86451-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86451-3-RR

    Affinity and Kinetic Characterization of 86451-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86451-3-RR against Human GRWD1 were performed. The affinity constant is 0.203 nM.

Geprüfte Anwendungen

Produktinformation

86451-3-PBS bindet in WB, IF/ICC, Indirect ELISA GRWD1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GRWD1 fusion protein Ag0367
Vollständiger Name glutamate-rich WD repeat containing 1
Berechnetes Molekulargewicht 49 kDa
Beobachtetes Molekulargewicht49-63 kDa
GenBank-ZugangsnummerBC002440
Gene symbol GRWD1
Gene ID (NCBI) 83743
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. (PMID:15885502)