GRWD1 Rekombinanter Antikörper

GRWD1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251273F6

Kat-Nr. : 86451-3-RR

Synonyme

Glutamate-rich WD repeat-containing protein 1, GRWD, KIAA1942, RRB1, WDR28

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  WB analysis using 86451-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86451-3-RR (GRWD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86451-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 86451-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRWD1 antibody (86451-3-RR, Clone: 251273F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86451-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86451-3-RR against Human GRWD1 were performed. The affinity constant is 0.203 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, NIH/3T3-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86451-3-RR bindet in WB, IF/ICC, ELISA GRWD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GRWD1 fusion protein Ag0367
• Vollständiger Name: glutamate-rich WD repeat containing 1
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49-63 kDa
• GenBank-Zugangsnummer: BC002440
• Gene symbol: GRWD1
• Gene ID (NCBI): 83743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. (PMID:15885502)