GS28 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

GS28 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242552G12

Kat-Nr. : 84971-3-PBS

Synonyme

242552G12, 28 kDa cis-Golgi SNARE p28, 28 kDa Golgi SNARE protein, Golgi SNAP receptor complex member 1, GOS-28

---

• 

  WB analysis using 84971-3-RR (same clone as 84971-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84971-3-RR (GS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 84971-3-RR (same clone as 84971-3-PBS)

  WB result of GS28 antibody (84971-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GS28 transfected HepG2 cells. This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 84971-3-RR (same clone as 84971-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84971-3-RR (same clone as 84971-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01719-2

  Cytometric bead array standard curve of MP01719-2, GS28 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84971-3-PBS. Detection antibody: 84971-4-PBS. Standard: Ag9021. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84971-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84971-3-RR against Human GS28 were performed. The affinity constant is 2.23 nM.

---

Geprüfte Anwendungen

Produktinformation

84971-3-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA GS28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GS28 fusion protein Ag9021
• Vollständiger Name: golgi SNAP receptor complex member 1
• Berechnetes Molekulargewicht: 28.6 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC012620
• Gene symbol: GS28
• Gene ID (NCBI): 9527
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)