GS28 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

GS28 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242552G12

Kat-Nr. : 84971-3-RR

Synonyme

242552G12, 28 kDa cis-Golgi SNARE p28, 28 kDa Golgi SNARE protein, Golgi SNAP receptor complex member 1, GOS-28

---

• 

  WB analysis using 84971-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84971-3-RR (GS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 84971-3-RR

  WB result of GS28 antibody (84971-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GS28 transfected HepG2 cells.

• 

  IHC staining of human stomach cancer using 84971-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 84971-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 84971-3-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 84971-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84971-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84971-3-RR against Human GS28 were performed. The affinity constant is 2.23 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, C6-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84971-3-RR bindet in WB, IHC, IF/ICC, ELISA GS28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GS28 fusion protein Ag9021
• Vollständiger Name: golgi SNAP receptor complex member 1
• Berechnetes Molekulargewicht: 28.6 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC012620
• Gene symbol: GS28
• Gene ID (NCBI): 9527
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)