GSK3B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSK3B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

304

Rezensionen (1)

Kat-Nr. : 22104-1-AP

Synonyme

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta, GSK3β

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Galerie der Validierungsdaten

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  WB analysis of A549 using 22104-1-AP

  WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

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  WB analysis using 22104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22104-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22104-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22104-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 22104-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 22104-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22104-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse brain using 22104-1-AP

  IP Result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GSK3B antibody 22104-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, LNCaP-Zellen, Maus-Eierstockgewebe, Maushirngewebe, Maushodengewebe, Mausnierengewebe, Maus-Thymusgewebe, PC-3-Zellen, Rattenhirngewebe, Ratten-Thymusgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hodengewebe, humanes Prostatahyperplasie-Gewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 293 publications below
• IHC: See 15 publications below
• IF: See 9 publications below
• IP: See 5 publications below
• CoIP: See 4 publications below
• RIP: See 1 publications below

Produktinformation

22104-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA GSK3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Huhn, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSK3B fusion protein Ag17320
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46-48 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution. This antibody recognize the C-terminal of GSK3B.

Publikationen

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha
human	WB	Mol Cancer; Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1.; Authors - Shan Xing
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
zebrafish	WB	Nat Commun; ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.; Authors - Xiaoyun Sun
rat	WB	Hepatology; Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.; Authors - Daimin Xiang
rat	WB	Theranostics; A functionalized self-assembling peptide containing E7 and YIGSR sequences enhances neuronal differentiation of spermatogonial stem cells on aligned PCL fibers for spinal cord injury repair; Authors - Zhiyuan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

HEK293 lysates - 10ug total protein. Blocked in 5% BSA before primary antibody (1:1000) overnight at 4 degrees. Anti-rabbit HRP (1:10,000) for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

GSK3B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:22104-1-AP)