GSK3B Polyklonaler Antikörper

GSK3B Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 24198-1-AP

Synonyme

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 24198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24198-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24198-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 24198-1-AP

  IP Result of anti-GSK3B (IP:24198-1-AP, 4ug; Detection:24198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human testis using 24198-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24198-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 24198-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 24198-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HeLa using 24198-1-AP

  1X10^6 HeLa cells were stained with .05ug GSK3B antibody (24198-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, PC-12-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mit Etacrynsäure behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

24198-1-AP bindet in WB, IP, IHC, IF, FC, ELISA GSK3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSK3B fusion protein Ag21438
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Cardiovasc Med; Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.; Authors - Dong Ma
human	WB	Tumour Biol; Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts.; Authors - Yumei Cai
human	WB	Medicine (Baltimore); Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts.; Authors - Yumei Cai
		Biomed Res Int; Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling.; Authors - Xueyun Chen