GSK3B Monoklonaler Antikörper

GSK3B Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

CloneNo.

2E9E4

Rezensionen (1)

Kat-Nr. : 67329-1-Ig

Synonyme

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

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  WB analysis using 67329-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67329-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67329-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67329-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSK3B antibody (67329-1-Ig, Clone: 2E9E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

67329-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA GSK3B und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GSK3B fusion protein Ag17320
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46-48 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Publikationen

Species	Application	Title
human	WB	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse	WB	Phytomedicine; Deoxyshikonin from Arnebiae Radix promotes hair growth by targeting the Wnt/β-catenin signaling pathway; Authors - Yingna Li
mouse	WB	J Ethnopharmacol; Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice; Authors - Yin OuYang
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
mouse	WB	Mol Neurobiol; Treadmill Exercise Modulates the Leptin/LepR/GSK-3β Signalling Pathway to Improve Leptin Sensitivity and Alleviate Neuroinflammation in High-Fat Diet-Fed APP/PS1 Mice; Authors - Juan Wang
human	WB,IF	J Inflamm Res; Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.; Authors - Wen-Ying Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rajni (Verified Customer) (07-23-2025)

used to detect gsk3b in A549 cells treated with IGF -1

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549