GSK3B Rekombinanter Antikörper

GSK3B Rekombinant Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4N21

Kat-Nr. : 82061-1-PBS

Synonyme

GSK3 beta, 4N21, EC:2.7.11.1, EC:2.7.11.26, GSK 3 beta

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  WB analysis using 82061-1-RR (same clone as 82061-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82061-1-RR (GSK3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82061-1-RR (same clone as 82061-1-PBS)

  IP result of anti-GSK3B (IP:82061-1-RR, 4ug; Detection:82061-1-RR 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82061-1-PBS bindet in WB, IHC, IP, Indirect ELISA GSK3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GSK3B fusion protein Ag17320
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: ~45 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.