GSNOR,ADH5 Monoklonaler Antikörper

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GSNOR,ADH5 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5E6E2

Kat-Nr. : 66193-1-PBS

Synonyme

ADH5, 5E6E2, ADH 3, ADH 5, ADH3

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  WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

  WB result of GSNOR,ADH5 antibody (66193-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66193-1-Ig (same clone as 66193-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66193-1-Ig (same clone as 66193-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66193-1-Ig (same clone as 66193-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66193-1-Ig (same clone as 66193-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66193-1-Ig (GSNOR,ADH5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66193-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GSNOR,ADH5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GSNOR,ADH5 fusion protein Ag9511
• Vollständiger Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Berechnetes Molekulargewicht: 374 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC014665
• Gene symbol: ADH5
• Gene ID (NCBI): 128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).