GSPT2 Polyklonaler Antikörper

GSPT2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12989-1-AP

Synonyme

eRF3b, G1 to S phase transition 2, GSPT2, GST2

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12989-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12989-1-AP (GSPT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12989-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12989-1-AP (GSPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12989-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12989-1-AP (GSPT2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MCF-7 using 12989-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 12989-1-AP (GSPT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

12989-1-AP bindet in WB, IHC, IF, ELISA GSPT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSPT2 fusion protein Ag3641
• Vollständiger Name: G1 to S phase transition 2
• Berechnetes Molekulargewicht: 628 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC036077
• Gene symbol: GSPT2
• Gene ID (NCBI): 23708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSPT2, also named as Eukaryotic peptide chain release factor GTP-binding subunit ERF3B or G1 to S phase transition protein 2 homolog, is a 628 amino acid protein, which belongs to the GTP-binding elongation factor family. ERF3 subfamily. GSPT2 localizes in the cytoplasm and is highly expressed in IUCC stage II colorectal cancer (CRC). GSPT2 is involved in translation termination in response to the termination codons UAA, UAG and UGA and may play a role as a potent stimulator of the release factor activity of ETF1. It may play a role in cell cycle progression.

Publikationen

Species	Application	Title
human, mouse	WB	Mol Cell; Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing.; Authors - Hong Su
		Nat Commun; Noninvasive urinary protein signatures associated with colorectal cancer diagnosis and metastasis.; Authors - Yulin Sun
mouse	WB	RNA; Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate.; Authors - Huang Kai-Lieh KL
		Int J Mol Med; eRF3b-37 inhibits the TGF-β1-induced activation of hepatic stellate cells by regulating cell proliferation, G0/G1 arrest, apoptosis and migration.; Authors - Zhengrong Xu
human	IF	Cells; Relocalization of Translation Termination and Ribosome Recycling Factors to Stress Granules Coincides with Elevated Stop-Codon Readthrough and Reinitiation Rates upon Oxidative Stress; Authors - Desislava S Makeeva