• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GSPT2 Rekombinanter Antikörper

GSPT2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251224C9

Kat-Nr. : 86508-3-PBS

Synonyme

EC:3.6.5.-, eRF3b, Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, G1 to S phase transition 2, GST2

Filter:
  • Western Blot (WB) analysis of various lysates using GSPT2 Recombinant antibody (86508-3-RR)

    WB analysis using 86508-3-RR (same clone as 86508-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86508-3-RR (GSPT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86508-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GSPT2 Recombinant antibody (86508-3-RR)

    IF Staining of MCF-7 using 86508-3-RR (same clone as 86508-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using GSPT2 antibody (86508-3-RR, Clone: 251224C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86508-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86508-3-RR

    Affinity and Kinetic Characterization of 86508-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86508-3-RR against Human GSPT2 were performed. The affinity constant is 2.18 nM.

Geprüfte Anwendungen

Produktinformation

86508-3-PBS bindet in WB, IF/ICC, Indirect ELISA GSPT2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GSPT2 fusion protein Ag3641
Vollständiger Name G1 to S phase transition 2
Berechnetes Molekulargewicht 628 aa, 69 kDa
Beobachtetes Molekulargewicht 69-75 kDa
GenBank-ZugangsnummerBC036077
Gene symbol GSPT2
Gene ID (NCBI) 23708
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSPT2, also named as eukaryotic peptide chain release factor GTP-binding subunit ERF3B or G1 to S phase transition protein 2 homolog, is a 628 amino acid protein, which belongs to the GTP-binding elongation factor family ERF3 subfamily. GSPT2 localizes in the cytoplasm and is highly expressed in IUCC stage II colorectal cancer (CRC). GSPT2 is involved in translation termination in response to the termination codons UAA, UAG, and UGA and may play a role as a potent stimulator of the release factor activity of ETF1. It may play a role in cell cycle progression.