GSPT2 Rekombinanter Antikörper

GSPT2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251224C9

Kat-Nr. : 86508-3-RR

Synonyme

EC:3.6.5.-, eRF3b, Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, G1 to S phase transition 2, GST2

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  WB analysis using 86508-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86508-3-RR (GSPT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86508-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using GSPT2 antibody (86508-3-RR, Clone: 251224C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86508-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86508-3-RR against Human GSPT2 were performed. The affinity constant is 2.18 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RKO cells, C6-Zellen, HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86508-3-RR bindet in WB, IF/ICC, ELISA GSPT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GSPT2 fusion protein Ag3641
• Vollständiger Name: G1 to S phase transition 2
• Berechnetes Molekulargewicht: 628 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69-75 kDa
• GenBank-Zugangsnummer: BC036077
• Gene symbol: GSPT2
• Gene ID (NCBI): 23708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSPT2, also named as eukaryotic peptide chain release factor GTP-binding subunit ERF3B or G1 to S phase transition protein 2 homolog, is a 628 amino acid protein, which belongs to the GTP-binding elongation factor family ERF3 subfamily. GSPT2 localizes in the cytoplasm and is highly expressed in IUCC stage II colorectal cancer (CRC). GSPT2 is involved in translation termination in response to the termination codons UAA, UAG, and UGA and may play a role as a potent stimulator of the release factor activity of ETF1. It may play a role in cell cycle progression.