GSS Monoklonaler Antikörper

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GSS Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

hamster, Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F12G1

Kat-Nr. : 67598-1-PBS

Synonyme

1F12G1, EC:6.3.2.3, Glutathione synthase, glutathione synthetase, GSH S

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  WB analysis using 67598-1-Ig (same clone as 67598-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67598-1-Ig (same clone as 67598-1-PBS)

  WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67598-1-Ig (same clone as 67598-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67598-1-PBS bindet in WB, IHC, IF/ICC, ELISA GSS und zeigt Reaktivität mit hamster, Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: hamster, Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GSS fusion protein Ag8354
• Vollständiger Name: glutathione synthetase
• Berechnetes Molekulargewicht: 474 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007927
• Gene symbol: GSS
• Gene ID (NCBI): 2937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.