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GST Tag Rekombinanter Antikörper

GST Tag Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

9B24

Kat-Nr. : 80006-1-PBS

Synonyme

GST, Glutathione S-Transferase

Filter:
  • Western Blot (WB) analysis of various lysates using GST Tag Recombinant antibody (80006-1-RR)

    WB analysis using 80006-1-RR (same clone as 80006-1-PBS)

    GST tagged protein, target band 42 kDa and degraded band 32 kDa, and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80006-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Recombinant protein using GST Tag Recombinant antibody (80006-1-RR)

    IP experiment of Recombinant protein using 80006-1-RR (same clone as 80006-1-PBS)

    IP result of anti-GST Tag (IP:80006-1-RR, 4ug; Detection:80006-1-RR 1:5000) with Recombinant protein lysate 1200 ug. This data was developed using the same antibody clone with 80006-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80006-1-PBS bindet in WB, IP, Indirect ELISA GST Tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GST Tag fusion protein Ag0040
Vollständiger Name GST Tag
Berechnetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerFN315687
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.