GST Tag Monoklonaler Antikörper

GST Tag Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

rekombinanten Protein, schistosoma japonicum und mehr (1)

Anwendung

WB, IP, CoIP

Konjugation

HRP Fluorescent Dye

Publikationen

27

CloneNo.

3G12B10

Kat-Nr. : HRP-66001

Synonyme

GST, GST tag

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Galerie der Validierungsdaten

• 

  WB analysis of Recombinant protein using HRP-66001

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66001 (GST Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:100000

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

HRP-66001 bindet in WB, IP, CoIP GST Tag und zeigt Reaktivität mit rekombinanten Protein, schistosoma japonicum

• Getestete Reaktivität: rekombinanten Protein, schistosoma japonicum
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: GST Tag
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: -
• GenBank-Zugangsnummer: FN315687
• Konjugation: HRP Fluorescent Dye
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publikationen

Species	Application	Title
		Nature; Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.; Authors - Zhaoyang Li
	WB	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
		Cell Res; Degradation of lipid droplets by chimeric autophagy-tethering compounds.; Authors - Yuhua Fu
human	CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
	WB	Nat Commun; PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.; Authors - Shifan Wang
		J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo

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