GSTA1 Monoklonaler Antikörper

GSTA1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1E1F4

Kat-Nr. : 66624-1-Ig

Synonyme

Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1

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  WB analysis of pig liver using 66624-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 66624-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66624-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66624-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 66624-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 66624-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse hepatocytes using 66624-1-Ig

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66624-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66624-1-Ig (GSTA1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, humanes Lebergewebe, humanes Hodengewebe, Mauslebergewebe, Rattennierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66624-1-Ig bindet in WB, IHC, IF/ICC, ELISA GSTA1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GSTA1 fusion protein Ag6084
• Vollständiger Name: glutathione S-transferase alpha 1
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC053578
• Gene symbol: GSTA1
• Gene ID (NCBI): 2938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
pig	WB	J Anim Physiol Anim Nutr (Berl); Tolerable upper intake level of iron damages the liver of weaned piglets.; Authors - Haoxuan Ding
human,mouse	WB	Int J Mol Sci; Upregulation of Hepatic Glutathione S-Transferase Alpha 1 Ameliorates Metabolic Dysfunction-Associated Steatosis by Degrading Fatty Acid Binding Protein 1; Authors - Jing Jiang