GSTA2 Rekombinanter Antikörper

GSTA2 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251223E2

Kat-Nr. : 86428-3-PBS

Synonyme

EC:2.5.1.18, Glutathione S-transferase A2, GST class-alpha member 2, GST gamma, GST HA subunit 2

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  WB analysis using 86428-3-RR (same clone as 86428-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86428-3-RR (GSTA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86428-3-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 86428-3-RR (same clone as 86428-3-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 86428-3-RR (GSTA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86428-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02440-1

  The mean GSTA2 concentration was determined to be 5.91 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 58.64 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02440-1

  Sandwich ELISA standard curve of MP02440-1, Human GSTA2 Recombinant Matched Antibody Pair - PBS only. 86428-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0781. 86428-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Cytometric bead array sample test of MP02440-1

  The mean GSTA2 concentration was determined to be 135.2 ng/mL in HepG2 cell extract based on a 1.1 mg/mL extract load.

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  Cytometric bead array standard curve of MP02440-1

  Cytometric bead array standard curve of MP02440-1, GSTA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86428-3-PBS. Detection antibody: 86428-2-PBS. Standard: Ag0781. Range: 0.781-50 ng/mL.

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  Affinity and Kinetic Characterization of 86428-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86428-3-RR against Human GSTA2 were performed. The affinity constant is 0.158 nM.

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Geprüfte Anwendungen

Produktinformation

86428-3-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA GSTA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GSTA2 fusion protein Ag0781
• Vollständiger Name: glutathione S-transferase alpha 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC002895
• Gene symbol: GSTA2
• Gene ID (NCBI): 2939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.