GSTA3 Polyklonaler Antikörper

GSTA3 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16703-1-AP

Synonyme

Glutathione S transferase A3, Glutathione S transferase A3 3, GST class alpha member 3, GSTA3, GSTA3 3, GTA3

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Galerie der Validierungsdaten

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  WB analysis using 16703-1-AP

  mouse testis tissue was subjected to SDS PAGE followed by western blot with 16703-1-AP (GSTA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16703-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16703-1-AP (GSTA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 16703-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16703-1-AP (GSTA3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16703-1-AP

  IP Result of anti-GSTA3 (IP:16703-1-AP, 4ug; Detection:16703-1-AP 1:500) with mouse testis tissue lysate 4800ug.

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  IF Staining of MCF-7 using 16703-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GSTA3 antibody 16703-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Hodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

16703-1-AP bindet in WB, IP, IF, ELISA GSTA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTA3 fusion protein Ag10101
• Vollständiger Name: glutathione S-transferase alpha 3
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC020619
• Gene symbol: GSTA3
• Gene ID (NCBI): 2940
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Front Pharmacol; PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization.; Authors - Mohammad Nasiruddin Rana
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
mouse	WB	Am J Pathol; Novel Protective Role of Nicotinamide Phosphoribosyltransferase in Acetaminophen-Induced Acute Liver Injury in Mice.; Authors - Li Qin Zhang
rat	WB	PLoS One; Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells.; Authors - Yoko Ito
mouse	WB	J Toxicol Pathol; Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice.; Authors - Shirai Makoto M