GSTA4 Polyklonaler Antikörper

GSTA4 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 17271-1-AP

Synonyme

EC:2.5.1.18, Glutathione S transferase A4, Glutathione S transferase A4 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4

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  WB analysis of human brain using 17271-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17271-1-AP (GSTA4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17271-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17271-1-AP (GSTA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 17271-1-AP

  IP result of anti-GSTA4 (IP:17271-1-AP, 4ug; Detection:17271-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

• 

  IF Staining of HEK-293 using 17271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 17271-1-AP (GSTA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

17271-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GSTA4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTA4 fusion protein Ag11028
• Vollständiger Name: glutathione S-transferase alpha 4
• Berechnetes Molekulargewicht: 222 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC015523
• Gene symbol: GSTA4
• Gene ID (NCBI): 2941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTA4 (Glutathione S-Transferase Alpha 4) is an enzyme that plays a key role in detoxification and antioxidant defense. Its structure includes a GSH-binding site and a hydrophobic substrate-binding domain, functioning as a dimer. Dysregulation of GSTA4 is linked to diseases like cancer, neurodegeneration, and diabetes.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB	Free Radic Biol Med; Increased hepatocellular protein carbonylation in human end-stage alcoholic cirrhosis.; Authors - C T Shearn
mouse	WB	Mech Ageing Dev; WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus; Authors - Jiahui Liu
mouse	WB	J Lipid Res; Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.; Authors - Rebecca L Smathers
		PLoS One; Short term feeding of a high fat diet exerts an additive effect on hepatocellular damage and steatosis in liver-specific PTEN knockout mice.; Authors - Colin T Shearn
human	WB	Exp Mol Pathol; Dysregulation of antioxidant responses in patients diagnosed with concomitant Primary Sclerosing Cholangitis/Inflammatory Bowel Disease.; Authors - Colin T Shearn

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iru (Verified Customer) (10-04-2021)

The antibody works well

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fallopian tube epithelial cells