GSTK1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTK1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (3)

Kat-Nr. : 14535-1-AP

Synonyme

DsbA-L, GST 13 13, GST, Glutathione S-transferase subunit 13, Glutathione S-transferase kappa 1

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  WB analysis of HepG2 using 14535-1-AP

  WB result of GSTK1 antibody (14535-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells.

• 

  WB analysis using 14535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14535-1-AP

  IP result of anti-GSTK1 (IP:14535-1-AP, 3ug; Detection:14535-1-AP 1:1000) with Raji cells lysate 2000ug.

• 

  IHC staining of human testis using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HUVEC-Zellen, L02-Zellen, Maushodengewebe, Raji-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14535-1-AP bindet in WB, IHC, IP, CoIP, ELISA GSTK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTK1 fusion protein Ag6036
• Vollständiger Name: glutathione S-transferase kappa 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC050715
• Gene symbol: GSTK1
• Gene ID (NCBI): 373156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

Publikationen

Species	Application	Title
mouse	WB	J Proteome Res; Proteomic Analysis of Hearts from Akita Mice Suggests Increases in Soluble Epoxide Hydrolase and Antioxidative Programming are Key Changes in Early Stages of Diabetic Cardiomyopathy.; Authors - Dewey Shannamar S
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
human	WB	Exp Mol Pathol; Aberrant expression of redox regulatory proteins in patients with concomitant primary Sclerosing cholangitis/inflammatory bowel disease.; Authors - Dennis R Petersen
human	CoIP	FASEB J; The role of chemerin in the regulation of cGAS-STING pathway in gestational diabetes mellitus placenta; Authors - Xuan Zhou
human,rat	WB	Sci Total Environ; m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance; Authors - Jingyuan Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (08-15-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

GSTK1 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:14535-1-AP)

FH

SD (Verified Customer) (07-05-2024)

Very good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

GSTK1 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:14535-1-AP)

FH

Andrea (Verified Customer) (01-23-2023)

Strong and specific signal.

• Applications: Western Blot