GSTK1 Polyklonaler Antikörper

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• KD/KO Validated

GSTK1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14535-1-PBS

Synonyme

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  WB analysis of HepG2 using 14535-1-AP (same clone as 14535-1-PBS)

  WB result of GSTK1 antibody (14535-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Raji using 14535-1-AP (same clone as 14535-1-PBS)

  IP result of anti-GSTK1 (IP:14535-1-AP, 3ug; Detection:14535-1-AP 1:1000) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14535-1-PBS bindet in WB, IHC, IP, Indirect ELISA GSTK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTK1 fusion protein Ag6036
• Vollständiger Name: glutathione S-transferase kappa 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC050715
• Gene symbol: GSTK1
• Gene ID (NCBI): 373156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).