GSTK1 Rekombinanter Antikörper

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• KD/KO Validated

GSTK1 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3J1

Kat-Nr. : 81527-1-PBS

Synonyme

DsbA-L, EC:2.5.1.18, Glutathione S-transferase kappa 1, Glutathione S-transferase subunit 13, GST

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  WB analysis using 81527-1-RR (same clone as 81527-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 81527-1-RR (same clone as 81527-1-PBS)

  WB result of GSTK1 antibody (81527-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 81527-1-RR (same clone as 81527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81527-1-RR (same clone as 81527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 81527-1-RR (same clone as 81527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81527-1-RR (same clone as 81527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81527-1-RR (same clone as 81527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81527-1-RR (same clone as 81527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 81527-1-RR (same clone as 81527-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81527-1-RR (same clone as 81527-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81527-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA GSTK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GSTK1 fusion protein Ag6036
• Vollständiger Name: glutathione S-transferase kappa 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC050715
• Gene symbol: GSTK1
• Gene ID (NCBI): 373156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity and hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).