GSTM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTM1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Rezensionen (1)

Kat-Nr. : 12412-1-AP

Synonyme

EC:2.5.1.18, glutathione S transferase mu 1, Glutathione S-transferase Mu 1, GST class mu 1, GST class-mu 1

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  WB analysis using 12412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12412-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12412-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12412-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12412-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, Mausnierengewebe, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

12412-1-AP bindet in WB, IHC, IF/ICC, ELISA GSTM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTM1 fusion protein Ag3113
• Vollständiger Name: glutathione S-transferase mu 1
• Berechnetes Molekulargewicht: 181 aa, 21 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC024005
• Gene symbol: GSTM1
• Gene ID (NCBI): 2944
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTM1 belongs to the superfamily of glutathione-Stransferases (GSTs) of phase 2 enzymes that catalyze a number of distinct glutathione-dependent reactions. GSTM1 gene has been a natural candidate in studies of susceptibility to inflammatory airway diseases with environmental components because of the high prevalence of the null polymorphism and its role in detoxification (PMID: 22683820).

Publikationen

Species	Application	Title
mouse	WB	Immunity; Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.; Authors - Yujia Wang ; KO Validated
human	WB	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang
human	IHC	Acta Neuropathol; Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy.; Authors - Woutje M Berdowski
mouse	WB	Sci Adv; lncRNA-GM targets Foxo1 to promote T cell-mediated autoimmunity; Authors - Yali Chen
human	WB	Autophagy; ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.; Authors - Zhiqiang Deng
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

Antibody works well at provided dilution in 1X TBST. Bands showed up around 25 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse liver tissue