GSTM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTM1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12412-1-PBS

Synonyme

EC:2.5.1.18, glutathione S transferase mu 1, Glutathione S-transferase Mu 1, GST class mu 1, GST class-mu 1

---

• 

  WB analysis using 12412-1-AP (same clone as 12412-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 12412-1-AP (same clone as 12412-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 12412-1-AP (same clone as 12412-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12412-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GSTM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTM1 fusion protein Ag3113
• Vollständiger Name: glutathione S-transferase mu 1
• Berechnetes Molekulargewicht: 181 aa, 21 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC024005
• Gene symbol: GSTM1
• Gene ID (NCBI): 2944
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTM1 belongs to the superfamily of glutathione-Stransferases (GSTs) of phase 2 enzymes that catalyze a number of distinct glutathione-dependent reactions. GSTM1 gene has been a natural candidate in studies of susceptibility to inflammatory airway diseases with environmental components because of the high prevalence of the null polymorphism and its role in detoxification (PMID: 22683820).