GSTM4 Polyklonaler Antikörper

GSTM4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16766-1-AP

Synonyme

GST class-mu 4, GST class mu 4, Glutathione S-transferase Mu 4, glutathione S transferase mu 4, EC:4.4.1.20

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  WB analysis of mouse liver using 16766-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16766-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16766-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 16766-1-AP

  IP result of anti-GSTM4 (IP:16766-1-AP, 4ug; Detection:16766-1-AP 1:500) with mouse lung tissue lysate 4000 ug.

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  IHC staining of human colon cancer using 16766-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16766-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16766-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16766-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16766-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTM4 antibody (16766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

16766-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GSTM4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTM4 fusion protein Ag10340
• Vollständiger Name: glutathione S-transferase mu 4
• Berechnetes Molekulargewicht: 218 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC015513
• Gene symbol: GSTM4
• Gene ID (NCBI): 2948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
human, mouse	WB	Mutat Res; Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in HepG2 cells and ICR mice.; Authors - Zhang Tianshun T
human	WB,IHC	Reprod Sci; 6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio) Hexanol Inhibits Proliferation and Induces Apoptosis of Endometriosis by Regulating Glutathione S-Transferase Mu Class 4; Authors - Wei Liu