GSTM5 Polyklonaler Antikörper

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• KD/KO Validated

GSTM5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 14502-1-AP

Synonyme

GSTM 5, GST class-mu 5, GST class mu 5, Glutathione S-transferase Mu 5, glutathione S transferase mu 5

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  WB analysis using 14502-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14502-1-AP (GSTM5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 14502-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14502-1-AP (GSTM5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 14502-1-AP

  IP result of anti-GSTM5 (IP:14502-1-AP, 4ug; Detection:14502-1-AP 1:600) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human heart using 14502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14502-1-AP (GSTM5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14502-1-AP (GSTM5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14502-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTM5 antibody (14502-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

14502-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GSTM5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTM5 fusion protein Ag5958
• Vollständiger Name: glutathione S-transferase mu 5
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC058881
• Gene symbol: GSTM5
• Gene ID (NCBI): 2949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTM5 belongs to the GST superfamily that metabolizes a broad range of xenobiotics and carcinogens. GSTM5 is expressed in brain, testis, lung and a lesser extent in heart.

Publikationen

Species	Application	Title
mouse	WB	J Proteomics; Quantitative proteomic analysis of oxaliplatin induced peripheral neurotoxicity.; Authors - Linlin Yang
rat	WB, IHC, IF	J Neurol Sci; Proteomic analysis of the hippocampus in naïve and ischemic-preconditioned rat.; Authors - Takayuki Nakajima
mouse	WB	Andrology; Thiol changes during epididymal maturation: a link to flagellar angulation in mouse spermatozoa?; Authors - Ijiri T W TW
rat,human	WB,IHC,IF	Cell Death Discov; IGF2BP2 regulates the inflammation of fibroblast-like synoviocytes via GSTM5 in rheumatoid arthritis; Authors - Yunyi Nan ; KD Validated
rat	WB	Drug Des Devel Ther; The Mechanism of Xuanyu Tongjing Decoction Regulating NOD/NFκB Pathway to Inhibit Ectopic Tissue Inflammation to Reduce Ovarian Damage in Rats with Ovarian Endometriosis; Authors - Weisen Fan