GSTT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTT1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 15838-1-AP

Synonyme

Glutathione transferase T1 1, GST class theta 1, GSTT1

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Galerie der Validierungsdaten

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  WB analysis of human brain using 15838-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15838-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15838-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15838-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15838-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HepG2-Zellen, Maushirngewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below

Produktinformation

15838-1-AP bindet in WB, IHC, ELISA GSTT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTT1 fusion protein Ag8588
• Vollständiger Name: glutathione S-transferase theta 1
• Berechnetes Molekulargewicht: 240 aa, 27 kDa
• Beobachtetes Molekulargewicht: 28 kDa, 30 kDa
• GenBank-Zugangsnummer: BC007065
• Gene symbol: GSTT1
• Gene ID (NCBI): 2952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

Publikationen

Species	Application	Title
		Cell Syst; Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.; Authors - Stephanie A Zlatic
mouse	WB	FASEB J; Gstm1/Gstt1 is essential for reducing cisplatin ototoxicity in CBA/CaJ mice.; Authors - Peipei Li ; KO Validated
hamster	IHC	Toxicol Sci; Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-dichloropropane in Male Syrian Hamsters and B6C3F1 Mice.; Authors - Min Wei
human	WB	Drug Metab Dispos; Evaluation of hepatic glutathione transferase Mu 1 and Theta 1 activities in humans and mice using genotype information.; Authors - Arakawa Shingo S
human	WB	Curr Issues Mol Biol; COPS5 Conferred the Platinum Resistance in Epithelial Ovarian Cancer; Authors - Ming-Wei Lin
mouse	IHC	Int J Clin Exp Pathol; Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.; Authors - Yasunori Sato