GSTZ1 Polyklonaler Antikörper

GSTZ1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 14889-1-AP

Synonyme

glutathione transferase zeta 1, GSTZ1, GSTZ1 1, MAAI, MAI, Maleylacetoacetate isomerase

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  WB analysis using 14889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14889-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 14889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 14889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

14889-1-AP bindet in WB, IHC, ELISA GSTZ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTZ1 fusion protein Ag6676
• Vollständiger Name: glutathione transferase zeta 1
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC001453
• Gene symbol: GSTZ1
• Gene ID (NCBI): 2954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutathione transferase zeta 1(GSTZ1) catalyzes the biotransformation of a range of α-haloacids, including dichloroacetic acid (DCA), and the penultimate step in the tyrosine degradation pathway. GSTZ1 is preferentially expressed in hepatocytes and renal proximal tubule cells where phenylalanine and tyrosine are catabolized(PMID: 16550164). This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
rat	WB	BMC Complement Med Ther; Integrated proteomics and metabolomics analysis of lumbar in a rat model of osteoporosis treated with Gushukang capsules; Authors - Ruohui Lin
human	WB	ACS Chem Biol; Chemical Proteomics with Novel Fully Functionalized Fragments and Stringent Target Prioritization Identifies the Glutathione-Dependent Isomerase GSTZ1 as a Lung Cancer Target; Authors - Yi Liao
human	WB	Anal Chem; Spike-In Proteome Enhances Data-Independent Acquisition for Thermal Proteome Profiling; Authors - Qiqi Wang