GTF2H2C Polyklonaler Antikörper

GTF2H2C Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17959-1-AP

Synonyme

GTF2H2C, GTF2H2D

---

Galerie der Validierungsdaten

• 

  WB analysis using 17959-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17959-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17959-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17959-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17959-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17959-1-AP

  IP result of anti-GTF2H2C (IP:17959-1-AP, 4ug; Detection:17959-1-AP 1:500) with HepG2 cells lysate 2240 ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17959-1-AP bindet in WB, IP, ELISA GTF2H2C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF2H2C fusion protein Ag12498
• Vollständiger Name: general transcription factor IIH, polypeptide 2C
• Berechnetes Molekulargewicht: 395 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC105980
• Gene symbol: GTF2H2C
• Gene ID (NCBI): 728340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.