TFII I Polyklonaler Antikörper

TFII I Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10499-1-AP

Synonyme

BAP 135, BAP135, BTK associated protein 135, BTKAP1, DIWS, GTF2I, GTFII I, IB291, SPIN, SRF Phox1 interacting protein, TFII I, WBS, WBSCR6

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Galerie der Validierungsdaten

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  WB analysis using 10499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10499-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10499-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10499-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10499-1-AP (TFII I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10499-1-AP

  Immunofluorescent analysis of Hela cells, using GTF2I antibody 10499-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 10499-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TFII I antibody (10499-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10499-1-AP bindet in WB, IHC, IF, FC, ELISA TFII I und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFII I fusion protein Ag0773
• Vollständiger Name: general transcription factor II, i
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC004472
• Gene symbol: GTF2I
• Gene ID (NCBI): 2969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer; Authors - Jing Chen
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang