GTF3C2 Polyklonaler Antikörper

GTF3C2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27494-1-AP

Synonyme

TFIIIC110, TFIIIC BETA, TF3C-beta, TF3C beta, KIAA0011

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  WB analysis using 27494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27494-1-AP (GTF3C2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27494-1-AP (GTF3C2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27494-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GTF3C2 antibody (27494-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27494-1-AP bindet in WB, IHC, IF/ICC, ELISA GTF3C2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF3C2 fusion protein Ag26666
• Vollständiger Name: general transcription factor IIIC, polypeptide 2, beta 110kDa
• Berechnetes Molekulargewicht: 101 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC020981
• Gene symbol: GTF3C2
• Gene ID (NCBI): 2976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTF3C2, also named as KIAA0011, is a 911 amino acid protein, which localizes in the nucleus. GTF3C2 is required for RNA polymerase III-mediated transcription. GTF3C2 is a component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. GTF3C2 may play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.

Publikationen

Species	Application	Title
human	WB	Hum Genet; Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder; Authors - Aiko Iwata-Otsubo