GTF3C4 Polyklonaler Antikörper

GTF3C4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17653-1-PBS

Synonyme

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  WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17653-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA GTF3C4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF3C4 fusion protein Ag11898
• Vollständiger Name: general transcription factor IIIC, polypeptide 4, 90kDa
• Berechnetes Molekulargewicht: 822 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC094774
• Gene symbol: GTF3C4
• Gene ID (NCBI): 9329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTF3C4, also named as TFIIIC90, is essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. It has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3.