GTPBP4 Polyklonaler Antikörper

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• KD/KO Validated

GTPBP4 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13897-1-PBS

Synonyme

NGB, GTP-binding protein NGB, GTP-binding protein 4, GTP binding protein NGB, GTP binding protein 4

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  WB analysis using 13897-1-AP (same clone as 13897-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 13897-1-AP (same clone as 13897-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13897-1-AP (same clone as 13897-1-PBS)

  IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:5000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

  IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 13897-1-AP (same clone as 13897-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using GTPBP4 antibody (13897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13897-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA GTPBP4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTPBP4 fusion protein Ag4859
• Vollständiger Name: GTP binding protein 4
• Berechnetes Molekulargewicht: 634 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC038975
• Gene symbol: GTPBP4
• Gene ID (NCBI): 23560
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.