GTSE1 Polyklonaler Antikörper

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GTSE1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21319-1-PBS

Synonyme

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  WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21319-1-AP (same clone as 21319-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21319-1-AP (same clone as 21319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21319-1-AP (same clone as 21319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 21319-1-AP (same clone as 21319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21319-1-PBS bindet in WB, IHC, Indirect ELISA GTSE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTSE1 fusion protein Ag13497
• Vollständiger Name: G-2 and S-phase expressed 1
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 66-77 kDa, 100 kDa
• GenBank-Zugangsnummer: BC006325
• Gene symbol: GTSE1
• Gene ID (NCBI): 51512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.