Uroguanylin Polyklonaler Antikörper

Uroguanylin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 18113-1-AP

Synonyme

GUCA2B, GCAP II, GCAP II Uroguanylin, Guanylate cyclase activator 2B, Guanylate cyclase C-activating peptide 2

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  WB analysis of mouse pancreas using 18113-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 18113-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 18113-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 18113-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 18113-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse small intestine using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18113-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Uroguanylin antibody (18113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, Maus-Kolongewebe, Maus-Dünndarmgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Nierengewebe, humanes Magengewebe, Mausnierengewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

18113-1-AP bindet in WB, IHC, IF/ICC, ELISA Uroguanylin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Uroguanylin fusion protein Ag12589
• Vollständiger Name: guanylate cyclase activator 2B (uroguanylin)
• Berechnetes Molekulargewicht: 112 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12-13 kDa
• GenBank-Zugangsnummer: BC093779
• Gene symbol: Uroguanylin
• Gene ID (NCBI): 2981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uroguanylin, also named as GUCA2B, is a candidate intestinal natriuretic hormone that controls the sodium and water balance between the intestine and the kidneys. It has been reported that the uroguanylin knockout mouse exhibits elevated blood pressure. Therefore, the GUCA2B gene is thought to be a susceptibility gene for essential hypertension (EH).

Publikationen

Species	Application	Title
mouse	IHC	Front Physiol; Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress.; Authors - Nobuhiko Ebisutani
human	WB	Hum Gene Ther; Gene Co-Expression and miRNA Regulation: A Path to Early Intervention in Colorectal Cancer; Authors - Jason C Huang
rat	IF	PLoS One; Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine; Authors - Natchayaporn Thonapan
human,mouse	WB,IF	Front Pharmacol; Brain-derived uroguanylin as a regulator of postprandial brown adipose tissue activation: a potential therapeutic approach for metabolic disorders; Authors - Nikola Habek