GUCY1A3 Polyklonaler Antikörper

GUCY1A3 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12605-1-AP

Synonyme

GC SA3, GCS alpha 1, GCS alpha 3, GUC1A3, GUCA3, GUCSA3, GUCY1A1, GUCY1A3

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12605-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12605-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 12605-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12605-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12605-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12605-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12605-1-AP

  IP Result of anti-GUCY1A3 (IP:12605-1-AP, 4ug; Detection:12605-1-AP 1:500) with mouse lung tissue lysate 3600ug.

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  IHC staining of human heart using 12605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12605-1-AP (GUCY1A3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12605-1-AP (GUCY1A3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Cerebellum-Gewebe, humanes Nierengewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12605-1-AP bindet in WB, IP, IHC, IF, ELISA GUCY1A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GUCY1A3 fusion protein Ag3293
• Vollständiger Name: guanylate cyclase 1, soluble, alpha 3
• Berechnetes Molekulargewicht: 690 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC028384
• Gene symbol: GUCY1A3
• Gene ID (NCBI): 2982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Hepatology; Single-cell transcriptomic analysis reveals a hepatic stellate cell-activation roadmap and myofibroblast origin during liver fibrosis.; Authors - Wu Yang
mouse	WB	Nat Commun; Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.; Authors - Andrea de la Fuente-Alonso
mouse	WB	Int J Mol Sci; Hypertensive Effect of Downregulation of the Opioid System in Mouse Model of Different Activity of the Endogenous Opioid System.; Authors - Dominik S Skiba
mouse	WB,IHC	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
	WB,IF	J Exp Med; Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injury; Authors - Hao He
mouse	WB	Front Pharmacol; Stimulation of soluble guanylate cyclase by vericiguat reduces skeletal muscle atrophy of mice following chemotherapy; Authors - Bo-Ang Hu