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GUF1 Rekombinanter Antikörper

GUF1 Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251449D6

Kat-Nr. : 86747-1-PBS

Synonyme

EC:3.6.5.-, EF-4, Elongation factor 4 homolog, GTPase GUF1, Ribosomal back-translocase

Filter:
  • Western Blot (WB) analysis of various lysates using GUF1 Recombinant monoclonal antibody (86747-1-RR)

    WB analysis using 86747-1-RR (same clone as 86747-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86747-1-RR (GUF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86747-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GUF1 Recombinant monoclonal antibody (86747-1-RR)

    IP experiment of HepG2 using 86747-1-RR (same clone as 86747-1-PBS)

    IP result of anti-GUF1 (IP:86747-1-RR, 4ug; Detection:86747-1-RR 1:2000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 86747-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86747-1-RR

    Affinity and Kinetic Characterization of 86747-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86747-1-RR against Human GUF1 were performed. The affinity constant is 9.10 pM.

Geprüfte Anwendungen

Produktinformation

86747-1-PBS bindet in WB, IP, Indirect ELISA GUF1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GUF1 fusion protein Ag11579
Vollständiger Name GUF1 GTPase homolog (S. cerevisiae)
Berechnetes Molekulargewicht 669 aa, 74 kDa
Beobachtetes Molekulargewicht68-70 kDa
GenBank-ZugangsnummerBC036768
Gene symbol GUF1
Gene ID (NCBI) 60558
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes.