GUSB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GUSB Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16332-1-AP

Synonyme

Beta G1, Beta glucuronidase, glucuronidase, beta, GUSB, MPS7

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 16332-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16332-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16332-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16332-1-AP (GUSB antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16332-1-AP (GUSB antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16332-1-AP (GUSB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16332-1-AP (GUSB Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HL-60-Zellen, K-562-Zellen, Mausherzgewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

16332-1-AP bindet in WB, IHC, ELISA GUSB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GUSB fusion protein Ag9453
• Vollständiger Name: glucuronidase, beta
• Berechnetes Molekulargewicht: 651 aa, 75 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC014142
• Gene symbol: GUSB
• Gene ID (NCBI): 2990
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The GUSB gene encodes beta-glucuronidase, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It is a tetrameric glycoprotein composed of identical subunits. GUSB plays an important role in the degradation of dermatan and keratan sulfates. GUSB has two isoforms with the molecular mass of 75 kDa and 69 kDa, and it always can be detected as 78 kDa, 60 kDa and 18 kDa. The 60 kDa and 18 kDa polypeptides are derived by nicking 78-kDa GUSB subunit at Val159-Gly160 (PMID: 1311180, 9268591).

Publikationen

Species	Application	Title
mouse	WB	Science; The adaptive immune system is a major driver of selection for tumor suppressor gene inactivation.; Authors - Timothy D Martin ; KO Validated
mouse	IHC	J Invest Dermatol; Distinct roles for Dectin-1 and Dectin-2 in skin wound healing and neutrophilic inflammatory responses.; Authors - Kenji Yamaguchi
human	WB	PLoS One; Changes in retinal pigment epithelium related to cigarette smoke: possible relevance to smoking as a risk factor for age-related macular degeneration.; Authors - Wang Ai Ling AL
mouse	WB	PLoS One; Mitochondrial dysfunction leads to deconjugation of quercetin glucuronides in inflammatory macrophages.; Authors - Ishisaka Akari A
mouse	WB	PLoS One; C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells.; Authors - Daiki Mori ; KO Validated