GYPC Polyklonaler Antikörper

GYPC Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18147-1-PBS

Synonyme

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  WB analysis of human blood using 18147-1-AP (same clone as 18147-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 18147-1-AP (GYPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 18147-1-AP (same clone as 18147-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GYPC antibody (18147-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18147-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA GYPC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GYPC fusion protein Ag12825
• Vollständiger Name: glycophorin C (Gerbich blood group)
• Berechnetes Molekulargewicht: 128 aa, 14 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC106051
• Gene symbol: GYPC
• Gene ID (NCBI): 2995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.