GYS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GYS1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 10566-1-AP

Synonyme

glycogen synthase 1 (muscle), GSY, GYS, GYS1

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Galerie der Validierungsdaten

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  WB analysis using 10566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10566-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 10566-1-AP

  IP Result of anti-GYS1 (IP:10566-1-AP, 4ug; Detection:10566-1-AP 1:300) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human liver using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10566-1-AP (GYS1 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HepG2 using 10566-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10566-1-AP (GYS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10566-1-AP

  Immunofluorescent analysis of HepG2 cells, using GYS1 antibody 10566-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 10566-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GYS1 antibody (10566-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Prostatakarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

10566-1-AP bindet in WB, IP, IHC, IF, FC, ELISA GYS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GYS1 fusion protein Ag0857
• Vollständiger Name: glycogen synthase 1 (muscle)
• Berechnetes Molekulargewicht: 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC007688
• Gene symbol: GYS1
• Gene ID (NCBI): 2997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
human	WB,IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu ; KD Validated
mouse	WB	Arterioscler Thromb Vasc Biol; Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy.; Authors - Hongming Zhu
rat	WB	Metabolism; Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat; Authors - Zejian Liu
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
mouse	WB,IF	J Lipid Res; Dynamic and differential regulation of proteins that coat lipid droplets in fatty liver dystrophic mice.; Authors - Hall Angela M AM