GYS2 Rekombinanter Antikörper

GYS2 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242595F10

Kat-Nr. : 85122-4-PBS

Synonyme

242595F10, EC:2.4.1.11, Glycogen synthase 2, glycogen synthase 2 (liver)

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  WB analysis using 85122-4-RR (same clone as 85122-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85122-4-RR (GYS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85122-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85122-4-RR (same clone as 85122-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide (positive control) and mouse skeletal muscle tissue slide (Negative control) using 85122-4-RR (GYS2 antibody) at dilution of 1:50000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85122-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85122-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85122-4-RR against Human GYS2 were performed. The affinity constant is 3.11 nM.

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Geprüfte Anwendungen

Produktinformation

85122-4-PBS bindet in WB, IHC, Indirect ELISA GYS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GYS2 fusion protein Ag18012
• Vollständiger Name: glycogen synthase 2 (liver)
• Berechnetes Molekulargewicht: 703 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC126310
• Gene symbol: GYS2
• Gene ID (NCBI): 2998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GYS2 (Glycogen Synthase 2) is a key enzyme involved in the synthesis of glycogen in the liver. It catalyzes the addition of glucose molecules to the growing glycogen chain, playing a crucial role in maintaining glucose homeostasis. GYS2 is essential for liver glycogen synthesis, which acts as a critical energy reserve for maintaining blood glucose levels, especially during fasting or prolonged exercise. Impairment of GYS2 function can lead to glycogen storage disease type 0 (GSD-0), characterized by hypoglycemia and liver glycogen deficiency. The GYS2 is highest expressed in the liver, and its expression in liver was significantly higher than other tissues. (PMID:39150597; PMID:37574425)