Granzyme A Polyklonaler Antikörper

Granzyme A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11288-1-AP

Synonyme

CTL tryptase, CTLA3, Fragmentin 1, GrA, Granzyme 1, Granzyme A, GZMA, H factor, Hanukkah factor, HF, HFSP

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Galerie der Validierungsdaten

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  WB analysis using 11288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11288-1-AP (Granzyme A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11288-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11288-1-AP (Granzyme A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11288-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11288-1-AP (Granzyme A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 11288-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11288-1-AP (Granzyme A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 11288-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11288-1-AP (Granzyme A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

11288-1-AP bindet in WB, IHC, IF, FC, ELISA Granzyme A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Granzyme A fusion protein Ag1818
• Vollständiger Name: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30, 60 kDa
• GenBank-Zugangsnummer: BC015739
• Gene symbol: GZMA
• Gene ID (NCBI): 3001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.

Publikationen

Species	Application	Title
mouse	IHC	Int J Biol Sci; Rapamycin and Low-dose IL-2 Mediate an Immunosuppressive Microenvironment to Inhibit Benign Prostatic Hyperplasia; Authors - Tianyu Cao
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	IHC	Immunopharmacol Immunotoxicol; Identification of discrepancy between CTLA4 expression and CTLA4 activation in gastric cancer.; Authors - Zhen Xiang
chicken	WB	Res Vet Sci; Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius.; Authors - Cui-Ying Chen
chicken	WB,IF,FC	Dev Comp Immunol; Perforin and granzyme A release as novel tool to measure NK cell activation in chickens; Authors - Adil Ijaz