Galc Polyklonaler Antikörper

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• KD/KO Validated

Galc Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 11991-1-AP

Synonyme

2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher

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Galerie der Validierungsdaten

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  WB analysis using 11991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 11991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 11991-1-AP

  IP Result of anti-Galc (IP:11991-1-AP, 4ug; Detection:11991-1-AP 1:300) with NIH/3T3 cells lysate 4000ug.

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  IHC staining of human gliomas using 11991-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11991-1-AP (Galc antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A549-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 7 publications below

Produktinformation

11991-1-AP bindet in WB, IP, IHC, IF, ELISA Galc und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galc fusion protein Ag3914
• Vollständiger Name: galactosylceramidase
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 30 kDa, 50 kDa
• GenBank-Zugangsnummer: BC086671
• Gene symbol: Galc
• Gene ID (NCBI): 14420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).

Publikationen

Species	Application	Title
human	IF	Dev Cell; NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.; Authors - Oliver B Davis
mouse	WB	Stem Cells; Multipotent Stromal Cells Alleviate Inflammation, Neuropathology, and Symptoms Associated with Globoid Cell Leukodystrophy in the Twitcher Mouse.; Authors - Scruggs Brittni A BA
mouse	IHC	Mol Ther; AAV-mediated expression of galactocerebrosidase in brain results in attenuated symptoms and extended life span in murine models of globoid cell leukodystrophy.; Authors - Rafi Mohammad A MA
mouse	IHC	Mol Ther; Extended normal life after AAVrh10-mediated gene therapy in the mouse model of Krabbe disease.; Authors - Rafi Mohammad A MA
		Glia; Ncx3 gene ablation impairs oligodendrocyte precursor response and increases susceptibility to experimental autoimmune encephalomyelitis.; Authors - Antonella Casamassa
mouse	WB	FASEB J; Polyunsaturated fatty acid deficiency affects sulfatides and other sulfated glycans in lysosomes through autophagy-mediated degradation.; Authors - Yaping Wang