Galc Polyklonaler Antikörper

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• KD/KO Validated

Galc Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11991-1-PBS

Synonyme

GALCERase, Galactosylceramide beta-galactosidase, Galactocerebroside beta-galactosidase, Galactocerebrosidase, Gacy

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  WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

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  WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 11991-1-AP (same clone as 11991-1-PBS)

  IP result of anti-Galc (IP:11991-1-AP, 4ug; Detection:11991-1-AP 1:300) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11991-1-AP (same clone as 11991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11991-1-AP (Galc antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 11991-1-AP (same clone as 11991-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Galc antibody (11991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11991-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA Galc und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galc fusion protein Ag3914
• Vollständiger Name: galactosylceramidase
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 30 kDa, 50 kDa
• GenBank-Zugangsnummer: BC086671
• Gene symbol: Galc
• Gene ID (NCBI): 14420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).