Galectin-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Galectin-1 Polyklonal Antikörper für IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, IF-P, ELISA

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-11858

Synonyme

Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1, Galaptin

---

• 

  IF Staining of rat colon using CL594-11858

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat colon using CL594-11858

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using CL594-11858

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Ratten-Kolongewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-11858 bindet in IF/ICC, IF-P, ELISA Galectin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin-1 fusion protein Ag2432
• Vollständiger Name: lectin, galactoside-binding, soluble, 1
• Berechnetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC020675
• Gene symbol: Galectin-1
• Gene ID (NCBI): 3956
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).