Galectin 10 Polyklonaler Antikörper

Galectin 10 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25225-1-AP

Synonyme

CLC, Charcot Leyden crystal protein, Charcot-Leyden crystal protein, Gal 10, Gal-10

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  WB analysis of HL-60 using 25225-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25225-1-AP (Galectin 10 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 25225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 25225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 25225-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 25225-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25225-1-AP (Galectin 10 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of THP-1 using 25225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Galectin 10 antibody (25225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of THP-1 using 25225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Galectin 10 antibody (25225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

25225-1-AP bindet in WB, IHC, IF/ICC, ELISA Galectin 10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin 10 fusion protein Ag18569
• Vollständiger Name: Charcot-Leyden crystal protein
• Berechnetes Molekulargewicht: 142 aa, 16 kDa
• Beobachtetes Molekulargewicht: 13-16 kDa
• GenBank-Zugangsnummer: BC119711
• Gene symbol: Galectin 10
• Gene ID (NCBI): 1178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectin-10 (Gal-10), also called Charcot-Leyden Crystal (CLC) protein, is a member of the galectin superfamily of galactoside-binding lectins and is most prominently expressed in human eosinophils, where it forms the classic hexagonal bipyramidal Charcot-Leyden crystals that are considered hallmarks of eosinophilic inflammatory reactions.

Publikationen

Species	Application	Title
human	IHC	Syst Biol Reprod Med; Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.; Authors - Genhai Zhu
human	IHC	Int J Surg Pathol; Langerhans Cell Histiocytosis With Unusual Hexagonal Crystals in Addition to Usual Charcot-Leyden Crystals. Report of a Patient With Possible Process of Crystal Formation and Clinical Significance of a "Necrotic" Change; Authors - Sayaka Ando
human	IF	Cells; CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.; Authors - Ssu-Ju Fu